Figure 2. Hsa_circ_0091994 knockdown inhibited cell viability of AGS and HGC-27. (A) The relative level of hsa_circ_0091994 in human gastric epithelial cell line GES-1 and in GC cell lines including AGS, MKN45, and HGC-27. **P<0.01, compared with GES-1; n = 3. (B) AGS and HGC-27 cells were administrated with shRNA-ctrl, shRNA-1 or shRNA-2 of hsa_circ_0091994 for 48 hr. The efficacy of hsa_circ_0091994 knockdown was evaluated by RT-qPCR. (C, D) The effect of shRNA-1 or shRNA-2 of hsa_circ_0091994 on the viability of AGS and HGC-27 cells were determined by CCK-8 assay. **P<0.01, compared with blank; n = 3.