Priority Research Paper Volume 13, Issue 21 pp 23876—23894

Alteration of mitochondrial homeostasis is an early event in a C. elegans model of human tauopathy

Cytoplasmic calcium chelation rescues motility defects in tauwt-expressing larvae. (A) Mitochondrial activity is increased in tauwt-expressing L4 nematodes upon 10 mM EGTA supplementation. Locomotion defects are ameliorated in both (B) L1 and (C) L4 tauwt-expressing nematodes in response to 10 mM EGTA treatment (n = 50; ***P t-test). (D) Representative images of wild type and tauwt-expressing nematodes tracks with or without 10 mM EGTA treatment. (E) Velocity assessment of wild type and tauwt-expressing nematodes with or without 10 mM EGTA treatment (n = 8; NS P > 0.05, *P t-test).

Figure 4. Cytoplasmic calcium chelation rescues motility defects in tauwt-expressing larvae. (A) Mitochondrial activity is increased in tauwt-expressing L4 nematodes upon 10 mM EGTA supplementation. Locomotion defects are ameliorated in both (B) L1 and (C) L4 tauwt-expressing nematodes in response to 10 mM EGTA treatment (n = 50; ***P < 0.0001; unpaired t-test). (D) Representative images of wild type and tauwt-expressing nematodes tracks with or without 10 mM EGTA treatment. (E) Velocity assessment of wild type and tauwt-expressing nematodes with or without 10 mM EGTA treatment (n = 8; NS P > 0.05, *P < 0.03; unpaired t-test).